›› 2012, Vol. 24 ›› Issue (2): 145-147.doi: 10.3969/j.issn.1004-616x.2012.02.015

• 检测研究 • Previous Articles     Next Articles

The effect of folic acid on genetic damage induced by ethanol in human lymphoblast cell line

CHEN Hai-yan，NI Juan，WANG Xu   

  1. 1. School of Life Sciences, Yunnan Normal University, Kunming 650092; 2. The Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650092, Yunnan, China
  • Received:2011-08-17 Revised:2011-11-30 Online:2012-03-30 Published:2012-03-30
  • Contact: WANG Xu

PDF (PC)

1099

Abstract: OBJECTIVE: The study focused on the effect of folic acid (FA) on genetic damage induced by ethanol (EtOH) in human lymphoblast cell line. METHODS：The human lymphoblast cell line GM12593 were cultured in RPMI-1640 medium modified with different concentrations of FA (20，200，2 000 nmol/L). Ethanol was added into the medium at the concentrations of 0.09%，0.36% and 1.34% (V/V) through the culture period. After 8 days culture，the cytokinesis-block micronucleus assay (CBMN) was used to evaluate frequencies of micronucleated binucleated cell (MNed BNC)，nuclear buds (BUD) and nucleoplasmic bridges (NPB) in binucleated cells. RESULTS：Ethanol significantly increased the frequencies of MNed BNC，BUD and NPB (P<0.01). A concentration of 2 000 nmol/L of FA reduced the genotoxic effects from ethanol significantly (P <0.01). CONCLUSION：Ethanol could induce DNA damage，folic acid was able to protect human from the genotoxicity induced by enthnol in vitro.

Key words: folic acid, ethanol, genetic damage, cytokinesis-block micronucleus cytome assay